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spsC [2018-05-17 18:27:44]
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spsC [2018-05-17 18:27:44]

spore coat polysaccharide synthesis
locus
BSU37890
pI
5.55
mw
43.03 kDa
protein length
389 aa Sequence Blast
gene length
1167 bp Sequence Blast
function
spore coat polysaccharide synthesis
product
unknown
essential
no
synonyms
ipa-65d
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.6|Miscellaneous metabolic pathways] → [category|SW 2.6.6.8|Biosynthesis of legionaminic acid (for spore crust))]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    3,889,732 → 3,890,901

    The protein

    Protein family

  • degT/dnrJ/eryC1 family (according to Swiss-Prot)

    • Structure

  • [PDB|1MDX] (ArnB from , 44% identity)

    • [SW|Localization]

  • binds the outer surface of the forming spore [Pubmed|14602648]

    • Expression and Regulation

    Operons

    genes
    [gene|219B0AAFB4ABB7E97A906553B73423F712505E27|spsA]-[gene|9E577B935FAB9605DAF83353E907A6561F11CAC1|spsB]-[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]-[gene|2648D9E356683F5E9C00C40F0A6BBD1933322B09|spsD]-[gene|F27921CD9B89EB16AA57ACECEDBF21ED383629F0|spsE]-[gene|222F7086A214A427174E188CB1FEE4A8B98D303C|spsF]-[gene|9B34C9DBEC5B84A631CDFBEE8DF44384FF5C03B0|spsG]-[gene|8A80A058F270C134891CBCE7EBD8D7A23797F599|spsI]-[gene|90D505CAA4FAAEB6A6AF5C278E70DF714FACB4DA|spsJ]-[gene|4A4C5E6C1DFD46BC3CF9AEBA3FC1577767FF8465|spsK]-[gene|F87238E9B22F967370971ABA4F911EFAC4F559B5|spsL]
    description
    [Pubmed|9353933]

    sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|26577401], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]
  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15383836], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]

    • regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|25239894,15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

    • regulation

  • expressed during [SW|sporulation] in the mother cell ([SW|SigE], [protein|search|SigK], [protein|search|GerE]) [Pubmed|26577401,25239894,15383836]

    • additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]
    • view in new tab

    Biological materials

    Mutant

  • CS218 (''[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]''::''aphA3'', available in [SW|Colin Harwood]'s and [SW|Jörg Stülke]'s labs) [Pubmed|27197833]
  • BKE37890 (Δ[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA
  • BKK37890 (Δ[gene|38DBA751456A0C0EC5D028939ADB8860ADA28F94|spsC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37890 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTCTTTGCGCTCCTATC, downstream forward: _UP4_GATATCGTAAAAGGGGCTGA

    • References

  • 9353933,15383836,14602648,25239894,26577401,27197833